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Product Name
PPP1CC Polyclonal Antibody
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Description
Polyclonal antibody to PPP1CC
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Tested applications
WB, IHC, ChIP
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Species reactivity
Human, Mouse, Rat
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Alternative names
PPP1CC antibody; PP-1G antibody; PP1C antibody; PPP1G antibody; protein phosphatase 1 catalytic subunit gamma antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200
CHIP 1:50 - 1:200 -
Validations
Western blot - PPP1CC Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PPP1CC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Western blot - PPP1CC Polyclonal Antibody
Western blot analysis of extracts from normal (control) and PPP1CC knockout HeLa cells, using PPP1CC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.
Immunohistochemistry - PPP1CC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PPP1CC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PPP1CC Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody at dilution of 1:100 (40x lens).
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Background
Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Dephosphorylates RPS6KB1. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"