PPM1D Polyclonal Antibody
Polyclonal antibody to PPM1D
Human, Mouse, Rat
PPM1D antibody; PP2C-DELTA antibody; WIP1 antibody; IDDGIP antibody; protein phosphatase 1D antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PPM1D (NP_003611.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - PPM1D Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PPM1D antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit ._Exposure time: 60s.
Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity).
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