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Product Name
POP4 Polyclonal Antibody
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Description
Polyclonal antibody to POP4
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
POP4 antibody; RPP29 antibody; ribonuclease P protein subunit p29 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POP4 (NP_006618.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - POP4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POP4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - POP4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat liver using POP4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POP4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using POP4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POP4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using POP4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POP4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat heart using POP4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POP4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human prostate using POP4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - POP4 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse liver using POP4 antibody at dilution of 1:100 (40x lens).
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Background
Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. May function with RPP38 to coordinate the nucleolar targeting and/or assembly of RNase P.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"