Product Name

POMP antibody

Documents

Description

POMP Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HepG2 cells, L02 cells. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human liver tissue, human skin tissue. Positive FC detected in HepG2 cells. Observed molecular weight by Western-blot: 16kd

Tested applications

ELISA, IF, IHC, WB, FC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

C13orf12 antibody; HSPC014 antibody; HSPC036 antibody; hUMP1 antibody; PNAS 110 antibody; POMP antibody; proteasome maturation protein antibody; Proteassemblin antibody; Protein UMP1 homolog antibody; UMP1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of POMP recombinant protein (Accession Number: NM_015932). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114057(POMP Antibody) at dilution of 1:2000

Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:114057(POMP Antibody) at dilution of 1:50

Immunofluorescent analysis of HepG2 cells, using POMP antibody Catalog No:114057 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

1X10^6 HepG2 cells were stained with 0.2ug POMP antibody (Catalog No:114057, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Background

POMP represents proteasome maturation protein

POMP antibody

Product Information

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