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Product Name
Human EED (His & GST Tag) recombinant protein
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Description
Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.
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Protein name
Polycomb protein EED
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Protein short names
WAIT1; EMBRYONIC ECTODERM DEVELOPMENT; HEED; ENSMUSG00000039373; EED; L7RN5; L(7)5RN; LUSK
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Uniprot ID
O75530
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Gene Name
EED
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human EED (O75530-1) (Met 1-Arg 441) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human EED/GST chimera consists of 678 amino acids and has a calculated molecular mass of 78 kDa. It migrates as an approximately 75-85 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 93 % as determined by SDS-PAGE
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Validations
Human EED / Embryonic Ectoderm Development Protein (His & GST Tag) SDS-PAGE
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