-
Product Name
EED antibody
- Documents
-
Description
EED Rabbit Polyclonal antibody. Positive IP detected in PC-3 cells. Positive WB detected in COLO 320 cells, HeLa cells, K-562 cells, PC-3 cells. Observed molecular weight by Western-blot: 50-68 kDa
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
EED antibody; embryonic ectoderm development antibody; HEED antibody; Polycomb protein EED antibody; WAIT 1 antibody; WAIT1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of EED recombinant protein (Accession Number: NM_001308007). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
-
Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:110309(EED antibody) at dilution of 1:1000
IP Result of anti-EED (IP:Catalog No:110309, 4ug; Detection:Catalog No:110309 1:500) with PC-3 cells lysate 3200ug.
-
Background
EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. EED has three isoforms, with the molecular weight of isoforms 1/2/3: 50/53/46 kDa.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"