Polybia-MPI peptide (TFA removed)

Polybia-MPI peptide (TFA removed)

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

IDWKKLLDAAKQIL-NH2

H-Ile-Asp-Trp-Lys-Lys-Leu-Leu-Asp-Ala-Ala-Lys-Gln-Ile-Leu-NH2

14

97.2%

C₇₈H₁₃₂N₂₀O₁₉

1654

872043-01-1

Synthetic

Powder

Have anti-biofilm and antifungal activities against C. albicans and C. glabrata (Wang K et al. 2014). A mast cell lytic peptide, chemotaxis for polymorphonucleated leukocytes (PMNL). Not toxic to rat erythrocytes. Helix in SDS or TFE. Replacement of residue 7, 8, or 9 by a proline led to reduced helicity as well as antitumor activity (Wang et al. 2008) Peptides 29: 963-968. Animal model:mouse: A single atom change (CO-NH2 to CS-NH2) of MPI improved peptide stability to serum proteases and better inhibited sarcoma xenograft tumor (S180) growth from 18 (WT) to 38% (mutant) using a Kunming mouse model (Zhang W et al. 2010 Peptides 31:1832-8). Updated 9/6/12; 3/2014 GW. Polybia-MPI has Antibacterial activity. Polybia-MPI was found in venom, social wasp, Polybia paulista.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Souza BM, Mendes MA, Santos LD, et al. Structural and functional characterization of two novel peptide toxins isolated from the venom of the social wasp Polybia paulista. Peptides. 2005;26(11):2157-64.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.