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Product Name
POLR2I antibody
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Description
POLR2I Rabbit Polyclonal antibody. Positive WB detected in L02 cells, HepG2 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 15 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hRPB14.5 antibody; POLR2I antibody; RNA polymerase II subunit B9 antibody; RPB14.5 antibody; RPB9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLR2I recombinant protein (Accession Number: NM_006233). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:50-1:500
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:114044(POLR2I antibody) at dilution of 1:500
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:114044(POLR2I Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
POLR2I represents polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"