POLR2F antibody

Cat.#: 114042

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Product Information

  • Product Name
    POLR2F antibody
  • Documents
  • Description
    POLR2F Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human cervical cancer tissue, human placenta tissue. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 14kd
  • Tested applications
    ELISA, WB, IF, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    HRBP14.4 antibody; POLR2F antibody; POLRF antibody; RPABC14.4 antibody; RPABC2 antibody; RPB14.4 antibody; RPB6 antibody; RPB6 homolog antibody; RPC15 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of POLR2F recombinant protein (Accession Number: NM_021974). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114042(POLR2F antibody) at dilution of 1:500

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114042(POLR2F antibody) at dilution of 1:500

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:114042(POLR2F Antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:114042(POLR2F Antibody) at dilution of 1:200 (under 40x lens)

    Immunofluorescent analysis of MCF-7 cells, using POLR2F antibody Catalog No:114042 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of MCF-7 cells, using POLR2F antibody Catalog No:114042 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Background
    POLR2F represents polymerase (RNA) II (DNA directed) polypeptide F

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"