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Product Name
POLDIP2 antibody
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Description
POLDIP2 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, Jurkat cells, K-562 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 38 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp586F1524 antibody; PDIP38 antibody; POLDIP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of POLDIP2 recombinant protein (Accession Number: NM_015584). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:114000(POLDIP2 antibody) at dilution of 1:500
Immunofluorescent analysis of Hela cells, using POLDIP2 antibody Catalog No:114000 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-POLDIP2 (IP:Catalog No:114000, 4ug; Detection:Catalog No:114000 1:1000) with HeLa cells lysate 1600ug.
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Background
POLDIP2(Polymerase delta-interacting protein 2) is also named as PDIP38, POLD4. It is as a unique positive regulator of Nox4 via its association with p22phox and together with Nox4, has profound effects on Rhodependent cytoskeletal remodeling. It is also as a regulator of cell division. It consists of 368 amino acids and has a predicted molecular mass of 42 kDa, with a potential signal peptide cleavage site after the first N-terminal 48 residues, which would result in a protein of 37 kDa(PMID:19574552). Endogenous human and rat POLDIP2 localized to the nucleus, plasma membrane, and cytoplasm(PMID:17623671).
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References
- Maga G, Crespan E, Markkanen E. DNA polymerase δ-interacting protein 2 is a processivity factor for DNA polymerase λ during 8-oxo-7,8-dihydroguanine bypass. Proceedings of the National Academy of Sciences of the United States of America. 110(47):18850-5. 2013.
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