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Product Name
Human PLA2G7/PAFAH (His Tag) recombinant protein
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Description
Modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. Has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids.
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Protein name
Platelet-activating factor acetylhydrolase
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Protein short names
PLA2G7; PAFAD; R75400; LP-PLA2; LDL-PLA2; PAFAH
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Uniprot ID
Q13093
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Gene Name
PLA2G7; PAFAH
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human PLA2G7 (Q13093-1) precursor (Met 1-Asn 441) was expressed, with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The secreted recombinant human PLA2G7 comprises 431 amino acids with a predicted molecular mass of 49.2 kDa. As a result of glycosylation, it migrates as an approximately 50-55 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 88 % as determined by SDS-PAGE
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Activity
Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2-Thio-PAF), in the presence of 5, 5’Dithiobis(2-nitrobenzoic acid) (DTNB).
The specific activity is >5000 pmoles/min/μg. -
Validations
Human PLA2G7 / PAFAH Protein (His Tag) SDS-PAGE
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