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Product Name
PLA2G4A Polyclonal Antibody
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Description
Polyclonal antibody to PLA2G4A
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
PLA2G4A antibody; PLA2G4 antibody; cPLA2 antibody; cPLA2-alpha antibody; cytosolic phospholipase A2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human PLA2G4A (NP_077734.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:500
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Validations
Western blot - PLA2G4A Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PLA2G4A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.
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Background
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"