Cart 2

PKLR antibody

Cat.#: 113856

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    PKLR antibody
  • Documents
  • Description
    PKLR Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, 293 cell, HEK-293 cells, HeLa cells, HepG2.MCF7 cells, human kidney tissue, human liver tissue, mouse heart tissue, mouse muscle/liver tissue, mouse skeletal muscle tissue, NIH/3T3 cells. Positive IHC detected in human lung cancer tissue, human breast cancer tissue. Observed molecular weight by Western-blot: 58 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    PK1 antibody; PKL antibody; PKLR antibody; PKR antibody; PKRL antibody; Pyruvate kinase 1 antibody; Pyruvate kinase isozymes R/L antibody; pyruvate kinase antibody; liver and RBC antibody; R type/L type pyruvate kinase antibody; Red cell/liver pyruvate kinase antibody; RPK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PKLR recombinant protein (Accession Number: XM_047422592). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    WB results of Catalog No:113856.

    WB results of Catalog No:113856.

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113856(PKLR antibody) at dilution of 1:1000

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113856(PKLR antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:113856(PKLR Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:113856(PKLR Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:113856(PKLR Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using Catalog No:113856(PKLR Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).
  • References
    • Takeda Y, Kang HS, Freudenberg J, DeGraff LM, Jothi R, Jetten AM. Retinoic acid-related orphan receptor γ (RORγ): a novel participant in the diurnal regulation of hepatic gluconeogenesis and insulin sensitivity. PLoS genetics. 10(5):e1004331. 2014.
    • Wang S, Chi X, Wei H. Influenza A virus-induced degradation of eukaryotic translation initiation factor 4B contributes to viral replication by suppressing IFITM3 protein expression. Journal of virology. 88(15):8375-85. 2014.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"