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Product Name
PKC iota antibody
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Description
PKC iota Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, MCF7 cells, MCF-7 cells. Positive IP detected in HeLa cells. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 67 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
aPKC lambda/iota antibody; DXS1179E antibody; PKC iota antibody; PKCI antibody; PRKC lambda/iota antibody; PRKCI antibody; Protein kinase C iota type antibody; protein kinase C antibody; iota antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PKC iota recombinant protein (Accession Number: BC022016). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113852(PRKCI antibody) at dilution of 1:500
IP Result of anti-PRKCI (IP:Catalog No:113852, 3ug; Detection:Catalog No:113852 1:500) with HeLa cells lysate 2500ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113852(PRKCI antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113852(PRKCI antibody) at dilution of 1:100 (under 40x lens)
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Background
PRKCI represents protein kinase C, iota
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"