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Product Name
PKC beta antibody
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Description
PKC beta Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, K-562 cells, mouse thymus tissue, rat brain tissue. Positive IHC detected in human pancreas cancer tissue, human breast cancer tissue, human stomach tissue. Observed molecular weight by Western-blot: 77 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
PKC B antibody; PKC beta antibody; PKCB antibody; PRKCB antibody; PRKCB1 antibody; PRKCB2 antibody; Protein kinase C beta type antibody; protein kinase C antibody; beta antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PKC beta recombinant protein (Accession Number: NM_002738). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113847(PRKCB antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113847(PRKCB antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:113847(PRKCB antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-PRKCB (IP:Catalog No:113847, 3ug; Detection:Catalog No:113847 1:1000) with mouse brain tissue lysate 4000ug.
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Background
PRKCB represents protein kinase C, beta
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References
- Luo W, Wang X, Zheng L. Brucine suppresses colon cancer cells growth via mediating KDR signalling pathway. Journal of cellular and molecular medicine. 17(10):1316-24. 2013.
- Zhang Y, Zhan Y, Zhang D. Eupolyphaga sinensis walker displays inhibition on hepatocellular carcinoma through regulating cell growth and metastasis signaling. Scientific reports. 4:5518. 2014.
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