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Product Name
PINX1 antibody
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Description
PINX1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A431 cells, Jurkat cells. Positive IHC detected in human skin tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ20565 antibody; LPTL antibody; LPTS antibody; PIN2 interacting protein 1 antibody; Pin2 interacting protein X1 antibody; PINX1 antibody; Protein 67 11 3 antibody; TRF1 interacting protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PINX1 recombinant protein (Accession Number: NM_017884). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113911(PINX1 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:113911(PINX1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human skin tissue slide using Catalog No:113911(PINX1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:113911(PINX1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PINX1, also named as LPTL, LPTS and Protein 67-11-3, is a Liver-related putative tumor suppressor. It belongs to the PINX1 family. PINX1 is a microtubule-binding protein essential for faithful chromosome segregation. It mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. PINX1 inhibits telomerase activity. It may inhibit cell proliferation and act as tumor suppressor. PINX1 has been identified as a critical telomerase inhibitor and proposed to be a putative tumor suppressor gene. Loss of PinX1 has been found in a large variety of malignancies, but the expression status in epithelial ovarian tumors has not been investigated.
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References
- Zhang B, Qian D, Ma HH. Anthracyclines disrupt telomere maintenance by telomerase through inducing PinX1 ubiquitination and degradation. Oncogene. 31(1):1-12. 2012.
- Liu JY, Qian D, He LR. PinX1 suppresses bladder urothelial carcinoma cell proliferation via the inhibition of telomerase activity and p16/cyclin D1 pathway. Molecular cancer. 12(1):148. 2013.
- Tian XP, Qian D, He LR. The telomere/telomerase binding factor PinX1 regulates paclitaxel sensitivity depending on spindle assembly checkpoint in human cervical squamous cell carcinomas. Cancer letters. 353(1):104-14. 2014.
- Cai MY, Zhang B, He WP. Decreased expression of PinX1 protein is correlated with tumor development and is a new independent poor prognostic factor in ovarian carcinoma. Cancer science. 101(6):1543-9. 2010.
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