PIN1 antibody

Cat.#: 113907

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Product Information

  • Product Name
    PIN1 antibody
  • Documents
  • Description
    PIN1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells. Positive IP detected in HepG2 cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human renal cell carcinoma tissue. Observed molecular weight by Western-blot: 18 kDa
  • Tested applications
    ELISA, WB, IHC, IP, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DOD antibody; PIN1 antibody; PPIase Pin1 antibody; Rotamase Pin1 antibody; UBL5 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PIN1 recombinant protein (Accession Number: NM_006221). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113907(PIN1 antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113907(PIN1 antibody) at dilution of 1:1000

    IP Result of anti-PIN1 (IP:Catalog No:113907, 4ug; Detection:Catalog No:113907 1:500) with HepG2 cells lysate 2400ug.

    IP Result of anti-PIN1 (IP:Catalog No:113907, 4ug; Detection:Catalog No:113907 1:500) with HepG2 cells lysate 2400ug.

    Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus.
  • References
    • Poolman TM, Farrow SN, Matthews L, Loudon AS, Ray DW. Pin1 promotes GR transactivation by enhancing recruitment to target genes. Nucleic acids research. 41(18):8515-25. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"