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Product Name
PIN1 antibody
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Description
PIN1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells. Positive IP detected in HepG2 cells. Positive IF detected in HEK-293 cells. Positive IHC detected in human renal cell carcinoma tissue. Observed molecular weight by Western-blot: 18 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DOD antibody; PIN1 antibody; PPIase Pin1 antibody; Rotamase Pin1 antibody; UBL5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PIN1 recombinant protein (Accession Number: NM_006221). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113907(PIN1 antibody) at dilution of 1:1000
IP Result of anti-PIN1 (IP:Catalog No:113907, 4ug; Detection:Catalog No:113907 1:500) with HepG2 cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human renal cell carcinoma tissue slide using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HEK-293 cells using Catalog No:113907(PIN1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus.
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References
- Poolman TM, Farrow SN, Matthews L, Loudon AS, Ray DW. Pin1 promotes GR transactivation by enhancing recruitment to target genes. Nucleic acids research. 41(18):8515-25. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"