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Product Name
PIK3R3 Polyclonal Antibody
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Description
Polyclonal antibody to PIK3R3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse
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Alternative names
PIK3R3 antibody; p55 antibody; p55-GAMMA antibody; p55PIK antibody; phosphoinositide-3-kinase regulatory subunit 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human PIK3R3
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - PIK3R3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using PIK3R3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunofluorescence - PIK3R3 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - PIK3R3 Polyclonal Antibody
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"