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Product Name
PIK3R3 Monoclonal Antibody
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Description
Monoclonal antibody to PIK3R3
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
PIK3R3 antibody; p55 antibody; p55-GAMMA antibody; p55PIK antibody; phosphoinositide-3-kinase regulatory subunit 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human PIK3R3
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - PIK3R3 Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using PIK3R3 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - PIK3R3 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using PIK3R3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - PIK3R3 Monoclonal Antibody
Immunohistochemistry of paraffin-embedded mouse testis using PIK3R3 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - PIK3R3 Monoclonal Antibody
Immunofluorescence analysis of SH-SY-5Y cells using PIK3R3 antibody .
Immunofluorescence - PIK3R3 Monoclonal Antibody
Immunofluorescence analysis of PC-3M cells using PIK3R3 antibody .
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Background
Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"