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Product Name
PICK1 antibody
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Description
PICK1 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue. Positive WB detected in mouse brain tissue, human brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PICK antibody; PICK1 antibody; PRKCA binding protein antibody; PRKCABP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PICK1 recombinant protein (Accession Number: NM_001039584). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113886(PICK1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113886(PICK1 antibody) at dilution of 1:50 (under 25x lens)
IP Result of anti-PICK1 (IP:Catalog No:113886, 4ug; Detection:Catalog No:113886 1:500) with mouse brain tissue lysate 4400ug.
Immunofluorescent analysis of HEK-293 cells using Catalog No:113886(PICK1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybrid system. PICK1 acts as a critical regulator of membrane receptors’ subcellular trafficking to modulate neural processes such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney and muscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release and glutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophic lateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-β signaling.
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References
- Zhao B, Wang Q, Du J, Luo S, Xia J, Chen YG. PICK1 promotes caveolin-dependent degradation of TGF-β type I receptor. Cell research. 22(10):1467-78. 2012.
- Blanque A, Repetto D, Rohlmann A. Deletion of KIBRA, protein expressed in kidney and brain, increases filopodial-like long dendritic spines in neocortical and hippocampal neurons in vivo and in vitro. Frontiers in neuroanatomy. 9:13. 2015.
- Zhang B, Cao W, Zhang F. Protein interacting with C alpha kinase 1 (PICK1) is involved in promoting tumor growth and correlates with poor prognosis of human breast cancer. Cancer science. 101(6):1536-42. 2010.
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