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Product Name
PI4K2A Polyclonal Antibody
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Description
Polyclonal antibody to PI4K2A
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
PI4K2A antibody; PI4KII antibody; PIK42A antibody; phosphatidylinositol 4-kinase type 2-alpha antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human PI4K2A
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:20 - 1:100 -
Validations
Western blot - PI4K2A Polyclonal Antibody
Western blot analysis of extracts of HL-60 cells, using PI4K2A antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence - PI4K2A Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using PI4K2A antibody . Blue: DAPI for nuclear staining.
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Background
Membrane-bound phosphatidylinositol-4 kinase (PI4-kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"