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Product Name
PHOX2B antibody
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Description
PHOX2B Rabbit Polyclonal antibody. Positive IF detected in Neuro-2a cells. Positive IHC detected in mouse brain tissue, neuroblastoma tissue. Positive WB detected in Neuro-2a cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 32 kDa
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Tested applications
ELISA, WB, IF, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
NBPhox antibody; Neuroblastoma Phox antibody; paired like homeobox 2b antibody; PHOX2B antibody; PHOX2B homeodomain protein antibody; PMX2B antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PHOX2B recombinant protein (Accession Number: NM_003924). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Neuro-2a cells were subjected to SDS PAGE followed by western blot with Catalog No:113862(PHOX2B Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:113862(PHOX2B Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded neuroblastoma tissue slide using Catalog No:113862(PHOX2B Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Neuro-2a cells using Catalog No:113862(PHOX2B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Paired mesoderm homeobox protein 2B (PHOX2B), also named as Neuroblastoma Phox, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. PHOX2B localizes in the nucleus and is expressed in neuroblastoma, brain and adrenal gland. PHOX2B plays a role in the development of several major noradrenergic neuron populations, including the locus coeruleus. PHOX2B has an association with congenital central hypoventilation syndrome.
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