Polyclonal antibody to Phospho-STAT2-Y690
STAT2 antibody; IMD44 antibody; ISGF-3 antibody; P113 antibody; STAT113 antibody; signal transducer and activator of transcription 2 antibody
Antigen: A synthetic phosphorylated peptide around Y690 of human STAT2 (NP_005410.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:1000
Western blot - Phospho-STAT2-Y690 pAb
Western blot analysis of extracts of HeLa cells, using Phospho-STAT2-Y690 antibody at 1:2000 dilution. HeLa cells were treated by IFN-u03b1 (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 1s.
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. Acts as a regulator of mitochondrial fission by modulating the phosphorylation of DNM1L at 'Ser-616' and 'Ser-637' which activate and inactivate the GTPase activity of DNM1L respectively.
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