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Product Name
phospho-RCC1(Ser12) antibody
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Description
phospho-RCC1(Ser12) Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 45-50kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cell cycle regulatory protein antibody; CHC1 antibody; RCC1 antibody; RCC1 I antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_001048194). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113830(phospho-CHC1(Ser12) antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113830(phospho-CHC1(Ser12) antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:113830(phospho-CHC1(Ser12) antibody) at dilution of 1:50 (under 40x lens)
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Background
CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser12) antigen.
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