Polyclonal antibody to Phospho-MYL9-S19
MYL9 antibody; LC20 antibody; MLC-2C antibody; MLC2 antibody; MRLC1 antibody; MYRL2 antibody; myosin light chain 9 antibody
Antigen: A synthetic phosphorylated peptide around S19 of human MYL9 (NP_006088.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-MYL9-S19 pAb
Western blot analysis of extracts of C6 cells, using Phospho-MYL9-S19 antibody at 1:1000 dilution. C6 cells were treated by CIP(20uL/400ul) at 37u2103 for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 90s.
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.
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