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Product Name
Phospho-MARCKS-S162 pAb
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Description
Polyclonal antibody to Phospho-MARCKS-S162
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
MARCKS antibody; 80K-L antibody; MACS antibody; PKCSL antibody; PRKCSL antibody; myristoylated alanine-rich C-kinase substrate antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding S162 of human MARCKS
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:100 - 1:200 -
Validations
Western blot - Phospho-MARCKS-S162 pAb
Western blot analysis of extracts from 293 cells, using Phospho-MARCKS-S162 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunofluorescence - Phospho-MARCKS-S162 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-MARCKS-S162 antibody .
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Background
MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"