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Product Name
Phospho-Histone H1.3-T17/Histone H1.4-T17 mAb
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Description
Monoclonal antibody to Phospho-Histone H1.3-T17/Histone H1.4-T17
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding T17 of human Histone H1.3
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - Phospho-Histone H1.3-T17/Histone H1.4-T17 mAb
Western blot analysis of extracts of various cells, using Phospho-Histone H1.3-T17/Histone H1.4-T17 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-Histone H1.3-T17/Histone H1.4-T17 mAb
Immunohistochemistry of paraffin-embedded mouse colon using Phospho-Histone H1.3-T17/Histone H1.4-T17 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - Phospho-Histone H1.3-T17/Histone H1.4-T17 mAb
Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-Histone H1.3-T17/Histone H1.4-T17 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - Phospho-Histone H1.3-T17/Histone H1.4-T17 mAb
Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H1.3-T17/Histone H1.4-T17 antibody .
Immunofluorescence - Phospho-Histone H1.3-T17/Histone H1.4-T17 mAb
Immunofluorescence analysis of CRC cells using Phospho-Histone H1.3-T17/Histone H1.4-T17 antibody .
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Background
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"