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Product Name
Anti-Histone H1.3 Rabbit antibody
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Description
Histone H1.3 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Histone H1.3
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/200
ICC/IF: 1/100
FC: 1/20
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Validations
Immunocytochemistry of Histone H1.3(green) in Hela cells using Histone H1.3 Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of Histone H1.3 in C6,3T3,Hela cell lysates using Histone H1.3 Rabbit pAb(1:1000 diluted).Predicted band size:22kDa.Observed band size:30kDa.
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Background
Swiss-Prot Acc.P16402.Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation .
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"