Polyclonal antibody to Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220
Antigen: A phospho specific peptide corresponding to residues surrounding S246 of human HDAC4
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:1000
IHC 1:50 - 1:100
Western blot - Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 pAb
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer. Deacetylates HSPA1A and HSPA1B at 'Lys-77' leading to their preferential binding to co-chaperone STUB1.
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