Polyclonal antibody to Phospho-EEF2K-S366
Human, Mouse, Rat
EEF2K antibody; HSU93850 antibody; eEF-2K antibody; eukaryotic elongation factor 2 kinase antibody
Antigen: A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
Western blot - Phospho-EEF2K-S366 pAb
Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37u2103 for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit .Exposure time: 15s.
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
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