-
Product Name
Phospho-DOK2-Y299 pAb
- Documents
-
Description
Polyclonal antibody to Phospho-DOK2-Y299
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human
-
Alternative names
DOK2 antibody; p56DOK antibody; p56dok-2 antibody; docking protein 2 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding Y299 of human DOK2
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:100 - 1:200 -
Validations
Western blot - Phospho-DOK2-Y299 pAb
Western blot analysis of extracts from Jurkat cells using Phospho-DOK2-Y299 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-DOK2-Y299 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-DOK2-Y299 antibody .
Immunofluorescence - Phospho-DOK2-Y299 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-DOK2-Y299 antibody .
-
Background
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"