Polyclonal antibody to Phospho-CDC25A-S76
Human, Mouse, Rat
CDC25A antibody; CDC25A2 antibody; cell division cycle 25A antibody
Antigen: A phospho specific peptide corresponding to residues surrounding S76 of human CDC25A
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Western blot - Phospho-CDC25A-S76 pAb
Western blot analysis of extracts from HeLa cells using phospho-CDC25A-S76 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-CDC25A-S76 pAb
Immunohistochemistry of paraffin-embedded human breast carcinoma using phospho-CDC25A-S76 antibody .
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
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