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Product Name
Phospho-ADD1-S726 pAb
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Description
Polyclonal antibody to Phospho-ADD1-S726
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
ADD1 antibody; ADDA antibody; adducin 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A phospho specific peptide corresponding to residues surrounding S726 of human ADD1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:100 - 1:200 -
Validations
Western blot - Phospho-ADD1-S726 pAb
Western blot analysis of extracts from HeLa cells using Phospho-ADD1-S726 antibody .Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.
Immunohistochemistry - Phospho-ADD1-S726 pAb
Immunohistochemistry of paraffin-embedded human lung carcinoma using Phospho-ADD1-S726 antibody .
Immunofluorescence - Phospho-ADD1-S726 pAb
Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-ADD1-S726 antibody .
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Background
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"