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Product Name
phospho(409/410)-TDP43 antibody
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Description
phospho(409/410)-TDP43 Rabbit Polyclonal antibody. Positive WB detected in human placenta tissue, human heart tissue, mouse heart tissue. Observed molecular weight by Western-blot: 45-50 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ALS10 antibody; TAR DNA binding protein antibody; TAR DNA binding protein 43 antibody; TARDBP antibody; TDP 43 antibody; TDP43 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_007375). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:113827(phospho(409/410)-TDP43 antibody) at dilution of 1:1000
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Background
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. This antibody detects phosphorylated forms of TDP-43 as well as the cleavage product of 20-30 kDa. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
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References
- Pelletier S, Gingras S, Howell S, Vogel P, Ihle JN. An early onset progressive motor neuron disorder in Scyl1-deficient mice is associated with mislocalization of TDP-43. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(47):16560-73. 2012.
- Dobson-Stone C, Luty AA, Thompson EM. Frontotemporal dementia-amyotrophic lateral sclerosis syndrome locus on chromosome 16p12.1-q12.2: genetic, clinical and neuropathological analysis. Acta neuropathologica. 125(4):523-33. 2013.
- Okamoto K, Fujita Y, Hoshino E. An autopsy case of familial amyotrophic lateral sclerosis with a TARDBP Q343R mutation. Neuropathology : official journal of the Japanese Society of Neuropathology. 35(5):462-8. 2015.
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