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Product Name
PHLPP antibody
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Description
PHLPP Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue. Positive WB detected in mouse brain tissue, HEK-293 cells, HeLa cells, mouse liver tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 185 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
hSCOP antibody; KIAA0606 antibody; PHLPP antibody; PHLPP1 antibody; PLEKHE1 antibody; SCOP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PHLPP recombinant protein (Accession Number: BC126277). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113825(PHLPP antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:113825(PHLPP Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using PHLPP antibody Catalog No:113825 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-PHLPP (IP:Catalog No:113825, 5ug; Detection:Catalog No:113825 1:1000) with mouse brain tissue lysate 5000ug.
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Background
PHLPP, also named as KIAA0931, PHLPPL, is a 1323 amino acid protein, which contains 22 LRR (leucine-rich) repeats, 1 PH domain and 1 PP2C-like domain. PHLPP expression is concentrated in the cytoplasm and nucleus. In colorectal cancer tissue, PHLPP expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but it is largely excluded from the crypt bases. PHLPP uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation.
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References
- Wen YA, Li X, Goretsky T, Weiss HL, Barrett TA, Gao T. Loss of PHLPP protects against colitis by inhibiting intestinal epithelial cell apoptosis. Biochimica et biophysica acta. 1852(10 Pt A):2013-23. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"