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Product Name
PHF2 antibody
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Description
PHF2 Rabbit Polyclonal antibody. Positive IHC detected in human cervical cancer tissue. Positive WB detected in Jurkat cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 150 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
GRC5 antibody; JHDM1E antibody; KIAA0662 antibody; PHD finger protein 2 antibody; PHF2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PHF2 recombinant protein (Accession Number: BC152437). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113811(PHF2 Antibody) at dilution of 1:300
IP Result of anti-PHF2 (IP:Catalog No:113811, 4ug; Detection:Catalog No:113811 1:500) with HeLa cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:113811(PHF2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
PHD finger protein 2 (PHF2), also known as JHDM1E (Jumonji C domain-containing histone demethylase 1E) or GRC5, is a 1101 amino acid protein, which contains one JmjC domain and one PHD-type zinc finger. PHF2 belongs to the JHDM1 histone demethylase family and localizes in the nucleus. PHF2 demethylases both histones and non-histione proteins. PHF2 is widely expressed in various tissues and exists as two alternative splicing isoforms. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction
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