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PHD1 antibody

Cat.#: 113802

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Product Information

  • Product Name
    PHD1 antibody
  • Documents
  • Description
    PHD1 Rabbit Polyclonal antibody. Positive IF detected in PC-3 cells. Positive WB detected in mouse testis tissue, HEK-293 cells, HeLa cells, human spleen tissue, mouse heart tissue, PC-3 cells. Observed molecular weight by Western-blot: 48kd
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DKFZp434E026 antibody; Egl nine homolog 2 antibody; EGLN2 antibody; EIT6 antibody; Estrogen induced tag 6 antibody; HIF PH1 antibody; HIF prolyl hydroxylase 1 antibody; HIFPH1 antibody; HPH 1 antibody; HPH 3 antibody; PHD1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of PHD1 recombinant protein (Accession Number: NM_053046). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IF: 1:10-1:100

  • Validations

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113802(EGLN2 antibody) at dilution of 1:1000

    mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113802(EGLN2 antibody) at dilution of 1:1000

    Immunofluorescent analysis of PC-3 cells using Catalog No:113802(EGLN2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of PC-3 cells using Catalog No:113802(EGLN2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    EGLN2, also named as PHD1, EIT6 and HPH-3, catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. EGLN2 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation.
  • References
    • Ohe R, Aung NY, Meng H. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leukemia & lymphoma. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"