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Product Name
PGD antibody
- Documents
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Description
PGD Rabbit Polyclonal antibody. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells. Positive IF detected in HepG2 cells. Positive IHC detected in human lymphoma tissue, human liver cancer tissue. Observed molecular weight by Western-blot: 53 kDa,45 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
6PGD antibody; PGD antibody; PGDH antibody; phosphogluconate dehydrogenase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PGD recombinant protein (Accession Number: NM_002631). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113783(PGD antibody) at dilution of 1:500
IP Result of anti-PGD (IP:Catalog No:113783, 4ug; Detection:Catalog No:113783 1:500) with HepG2 cells lysate 3200ug.
Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:113783(PGD Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:113783(PGD Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:113783(PGD Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PGD represents phosphogluconate dehydrogenase
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"