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Product Name
PFKP antibody
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Description
PFKP Rabbit Polyclonal antibody. Positive IF detected in BxPC-3 cells. Positive IHC detected in human spleen tissue, human heart tissue, human lung cancer tissue. Positive WB detected in mouse heart tissue, BxPC-3 cells, HEK-293 cells, HeLa cells, human heart tissue, Jurkat cells, mouse skeletal muscle tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 86 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
6 phosphofructokinase type C antibody; FLJ40226 antibody; PFK C antibody; PFKF antibody; PFKP antibody; Phosphofructokinase 1 antibody; phosphofructokinase antibody; platelet antibody; Phosphohexokinase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PFKP recombinant protein (Accession Number: NM_002627). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113773(PFKP antibody) at dilution of 1:1500
IP Result of anti-PFKP (IP:Catalog No:113773, 4ug; Detection:Catalog No:113773 1:1000) with mouse heart tissue lysate 4000ug.
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:113773(PFKP Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human spleen tissue slide using Catalog No:113773(PFKP Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of BxPC-3 cells using Catalog No:113773(PFKP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
PFKP(6-phosphofructokinase, platelet type) is also named as PFKF,PFK-C and belongs to the phosphofructokinase family.This form of PFK is best called the 'platelet' type and symbolized PFKP because it is the only form made by platelets, whereas fibroblasts have more than one form of PFK(Francke (1983)).PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"