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Product Name
peroxiredoxin 2 antibody
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Description
peroxiredoxin 2 Mouse Monoclonal antibody. Positive WB detected in human testis tissue, fetal human brain tissue, HEK-293 cells, MCF-7 cells. Positive IHC detected in human testis tissue, human brain tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
NKEF B antibody; NKEFB antibody; peroxiredoxin 2 antibody; PRDX2 antibody; PRP antibody; PRX2 antibody; PRXII antibody; TDPX1 antibody; Thioredoxin peroxidase 1 antibody; TSA antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of peroxiredoxin 2 recombinant protein (Accession Number: NM_005809). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human testis using Catalog No:107464(PRDX2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:107464(PRDX2 antibody) at dilution of 1:50 (under 40x lens)
human testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:107464(PRDX2 Antibody) at dilution of 1:2000
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Background
PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)
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