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Product Name
Perforin antibody
- Documents
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Description
Perforin Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, mouse liver tissue. Observed molecular weight by Western-blot: 70-75 kDa
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Tested applications
ELISA, WB
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
Cytolysin antibody; FLH2 antibody; HPLH2 antibody; Perforin antibody; Perforin 1 antibody; PFN1 antibody; PFP antibody; PRF1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Perforin recombinant protein (Accession Number: NM_001083116). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113714(PRF1 antibody) at dilution of 1:500
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Background
Perforin 1 (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)
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References
- Chen CY, Xie QM, Xue Y. Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius. Research in veterinary science. 94(3):496-503. 2013.
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