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Product Name
PER3 antibody
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Description
PER3 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 132 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GIG13 antibody; hPER3 antibody; PER3 antibody; period homolog 3 (Drosophila) antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PER3 recombinant protein (Accession Number: BC026102). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113713(PER3 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113713(PER3 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:113713(PER3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The suprachiasmatic nuclei (SCN) of the anterior hypothalamus function as a circadian clock in the mammalian brain, generating circadian rhythms in physiology and behavior. The period homolog genesPer3 is an important component of the circadian clock system. PER3 plays a role in cell proliferation, apoptosis,DNA damage response, cell cycle control, and treatment response of chemotherapy agents in cancers.
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