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Product Name
PELI1 antibody
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Description
PELI1 Rabbit Polyclonal antibody. Positive IP detected in SH-SY5Y cells. Positive WB detected in mouse thymus tissue. Observed molecular weight by Western-blot: 46 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp686C18116 antibody; PELI1 antibody; Pellino 1 antibody; pellino homolog 1 (Drosophila) antibody; PRISM antibody; Protein pellino homolog 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PELI1 recombinant protein (Accession Number: BC011419). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:113650(PELI1 antibody) at dilution of 1:400
IP Result of anti-PELI1 (IP:Catalog No:113650, 4ug; Detection:Catalog No:113650 1:300) with SH-SY5Y cells lysate 3000ug.
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Background
PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation. This antibody may cross react with Pellino-3.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"