Product Name

PDK3 antibody

Documents

Description

PDK3 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human skeletal muscle tissue. Positive WB detected in mouse heart tissue, mouse kidney tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 47 kDa

Tested applications

ELISA, WB, IHC, IP

Species reactivity

Human, Mouse; other species not tested.

Alternative names

PDK3 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of PDK3 recombinant protein (Accession Number: NM_005391). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

Validations

mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113626(PDK3 antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:113626(PDK3 Antibody) at dilution of 1:50

IP Result of anti-PDK3 (IP:Catalog No:113626, 4ug; Detection:Catalog No:113626 1:1000) with mouse heart tissue lysate 4000ug.

Background

PDK3([Pyruvate dehydrogenase [lipoamide]] kinase isozyme 3, mitochondrial) is also named as PDHK3 and belongs to the PDK/BCKDK protein kinase family. It inhibits pyruvate dehydrogenase activity by phosphorylation of the E1 subunit PDHA1, and thereby regulates glucose metabolism and aerobic respiration. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 and 48 kDa. This protein can exsit as a dimer with the molecular weight of 90 kDa(PMID:11978179).

References

Izquierdo-Garcia JL, Viswanath P, Eriksson P. IDH1 Mutation Induces Reprogramming of Pyruvate Metabolism. Cancer research. 75(15):2999-3009. 2015.

PDK3 antibody

Product Information

Related Products / Services