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Product Name
PDK1 antibody
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Description
PDK1 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HEK-293 cells, NIH/3T3 cells, rat kidney tissue. Positive IHC detected in human heart tissue. Observed molecular weight by Western-blot: 49 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PDH kinase 1 antibody; PDHK1 antibody; PDK1 antibody; Pyruvate dehydrogenase kinase isoform 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDK1 recombinant protein (Accession Number: NM_002610). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113624(PDK1 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:113624(PDK1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human heart slide using Catalog No:113624(PDK1 Antibody) at dilution of 1:50
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Background
PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family.It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism(PMID:17683942).Starvation increases islet PDK activity(PMID:11723055).This antibody is speicfic to PDK1.
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