Product Name

PDI antibody

Documents

Description

PDI Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue, human lung cancer tissue. Positive IF detected in HEK-293 cells, HepG2 cells. Positive FC detected in HEK-293T cells. Positive IP detected in mouse liver tissue. Positive WB detected in COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, human thyroid gland tissue, mouse heart tissue, mouse thymus tissue, rat brain tissue, rat heart tissue, rat thymus tissue. Observed molecular weight by Western-blot: 57 kDa

Tested applications

ELISA, WB, IHC, IF, FC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

DSI antibody; ERBA2L antibody; GIT antibody; P4HB antibody; P4Hbeta antibody; p55 antibody; PDI antibody; PDIA1 antibody; PHDB antibody; PO4DB antibody; PO4HB antibody; PROHB antibody; Protein disulfide isomerase antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of PDI recombinant protein (Accession Number: BC014504). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IP: 1:200-1:1000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:113681(PDIA1 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113681(PDIA1 antibody) at dilution of 1:50 (under 40x lens)

1X10^6 HEK-293T cells were stained with .2ug PDI antibody (Catalog No:113681, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

IP Result of anti-PDI (IP:Catalog No:113681, 3ug; Detection:Catalog No:113681 1:300) with mouse liver tissue lysate 4000ug.

Immunofluorescent analysis of HEK-293 cells using Catalog No:113681(PDI Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Background

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

References

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Spector I, Zilberstein Y, Lavy A, Nagler A, Genin O, Pines M. Involvement of host stroma cells and tissue fibrosis in pancreatic tumor development in transgenic mice. PloS one. 7(7):e41833. 2012.
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Meissner M, Weissgerber P, Londoño JE. Moderate calcium channel dysfunction in adult mice with inducible cardiomyocyte-specific excision of the cacnb2 gene. The Journal of biological chemistry. 286(18):15875-82. 2011.
Wang SM, Lin LZ, Zhou DH, Zhou JX, Xiong SQ. Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines. Chinese journal of integrative medicine. 21(9):689-96. 2015.
Bodanovsky A, Guttman N, Barzilai-Tutsch H. Halofuginone improves muscle-cell survival in muscular dystrophies. Biochimica et biophysica acta. 1843(7):1339-47. 2014.
Smith CL, Baek ST, Sung CY, Tallquist MD. Epicardial-derived cell epithelial-to-mesenchymal transition and fate specification require PDGF receptor signaling. Circulation research. 108(12):e15-26. 2011.

PDI antibody

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