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Product Name
PDHA1 antibody
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Description
PDHA1 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse kidney tissue. Positive WB detected in mouse brain tissue, HeLa cells, mouse skeletal muscle tissue, rat brain tissue. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PDH E1 alpha antibody; PDHA1 antibody; PDHE1 A type I antibody; pyruvate dehydrogenase E1 α subunit antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDHA1 recombinant protein (Accession Number: NM_000284). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113678(PDHA1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human heart using Catalog No:113678(PDHA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113678(PDHA1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using PDHA1 antibody Catalog No:113678 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-PDHA1 (IP:Catalog No:113678, 4ug; Detection:Catalog No:113678 1:1000) with mouse kidney tissue lysate 4400ug.
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Background
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
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References
- Zhang J, Fan J, Venneti S. Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion. Molecular cell. 56(2):205-18. 2014.
- Liu F, Zhang W, You X. The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer. Oncotarget. 6(29):27199-213. 2015.
- Du J, Ma M, Zhao Q. Mitochondrial bioenergetic deficits in the hippocampi of rats with chronic ischemia-induced vascular dementia. Neuroscience. 231:345-52. 2013.
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