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Product Name
PDHA1 antibody
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Description
PDHA1 Mouse Monoclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue, human heart tissue. Positive WB detected in HEK-293 cells, HepG2 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 43 kDa
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
PDHA1 antibody; PDHE1 A type I antibody; pyruvate dehydrogenase E1 α subunit antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of PDHA1 recombinant protein (Accession Number: NM_000284). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IP: 1:5000-1:50000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107461(PDHA1 antibody) at dilution of 1:20000
IP Result of anti-PDHA1 (IP:Catalog No:107461, 3ug; Detection:Catalog No:107461 1:10000) with HEK-293 cells lysate 1800ug.
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107461(PDHA1 Antibody) at dilution of 1:50(under 10x lens)
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:107461(PDHA1 Antibody) at dilution of 1:50(under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:107461(PDHA1 Antibody) at dilution of 1:100 and Rhodamine-Goat anti-Mouse IgG
1X10^6 HepG2 cells were stained with .2ug PDHA1 antibody (Catalog No:107461, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).
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References
- Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.
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