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Product Name
Anti-Pyruvate Dehydrogenase E1-alpha subunit Rabbit antibody
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Description
Pyruvate Dehydrogenase E1-alpha subunit Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
PDHA; PDHAD; PHE1A; PDHCE1A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Pyruvate Dehydrogenase E1-alpha subunit
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000-1/2000
IHC: 1/20-1/100
ICC/IF: 1/20-1/100
FC: 1/20
IP: 1/20-1/50
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Validations
Western blot detection of Pyruvate Dehydrogenase E1-alpha subunit in C6,3T3 cell lysates using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:43kDa.
Immunocytochemistry of Pyruvate Dehydrogenase E1-alpha subunit(green) in Hela cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.P08559.The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"