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Product Name
PDGFRL antibody
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Description
PDGFRL Rabbit Polyclonal antibody. Positive WB detected in L02 cells, mouse liver tissue. Positive FC detected in HepG2 cells. Positive IHC detected in human liver cancer tissue, mouse liver tissue. Positive IF detected in L02 cells. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PDGFR like protein antibody; PDGFRL antibody; PDGRL antibody; PRLTS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PDGFRL recombinant protein (Accession Number: NM_001372073). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:113677(PDGFRL Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:113677(PDGFRL Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using Catalog No:113677(PDGFRL Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of L02 cells using Catalog No:113677(PDGFRL Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with .2ug PDGFRL antibody (Catalog No:113677, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
PDGFRL represents platelet-derived growth factor receptor-like
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